百里香酚对脂多糖诱导的子宫内膜上皮细胞炎性反应的抑制作用研究

doi:10.11843/j.issn.0366-6964.2019.07.019

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (7): 1493-1499.doi: 10.11843/j.issn.0366-6964.2019.07.019

百里香酚对脂多糖诱导的子宫内膜上皮细胞炎性反应的抑制作用研究

邵丹¹, 宋朋杰¹, 闫宝琪¹, 武小虎¹, 王东升¹, 李亚娟^1,2, 张世栋^1*, 严作廷^1*

1. 中国农业科学院兰州畜牧与兽药研究所, 农业部兽用药物创制重点实验室, 甘肃省新兽药工程重点实验室, 兰州 730050;
2. 广西大学动物科学技术学院, 南宁 530004

收稿日期:2019-01-03 出版日期:2019-07-23 发布日期:2019-07-23
通讯作者: 严作廷,主要从事奶牛疾病诊断和防控及中兽药研发,E-mail:yanzuoting@caas.cn;张世栋,主要从事奶牛疾病分子病理学研究,E-mail:zhangshidong@caas.cn
作者简介:邵丹(1993-),女,山东菏泽人,硕士,主要从事奶牛疾病诊断治疗的研究,E-mail:shaodan0101@163.com
基金资助:
“十三五”国家重点研发课题（2017YFD0502201）；中国农业科学院科技创新工程（CAAS-ASTIP-2014-LIHPS-03）

Inhibiting Effect of Thymol on Inflammation Induced by LPS in Endometrial Epithelial Cells

SHAO Dan¹, SONG Pengjie¹, YAN Baoqi¹, WU Xiaohu¹, WANG Dongsheng¹, LI Yajuan^1,2, ZHANG Shidong^1*, YAN Zuoting^1*

1. Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China;
2. Department of Veterinary Medicine, College of Animal Science and Technology, Guangxi University, Nanning 530004, China

Received:2019-01-03 Online:2019-07-23 Published:2019-07-23
Supported by:

摘要/Abstract

摘要： 为探究百里香酚治疗子宫内膜炎的抗炎活性，利用脂多糖（LPS）诱导山羊子宫内膜上皮细胞（gEECs）建立炎性模型，以百里香酚进行干预。采用NO试剂盒和酶联免疫吸附试验（ELISA）分别检测百里香酚对LPS诱导的NO和细胞因子分泌的影响；利用实时荧光定量PCR（RT-PCR）检测百里香酚对LPS诱导的IL-1β、TLR4和NF-κB基因转录的影响。结果显示：不同剂量的百里香酚（100、50、25 μg·mL^-1）能不同程度地抑制LPS诱导的炎性模型细胞TNF-α、IL-1β、IL-6、NO和PGE2的分泌，亦可降低炎性模型细胞IL-1β、TLR4和NF-κB的基因转录水平（P<0.01）。结果表明，百里香酚可抑制LPS诱导gEECs炎性模型的细胞因子表达水平，具有显著的抗炎活性，其作用机制可能与抑制TLR4/NF-κB信号通路相关。

Abstract: In order to investigate the anti-inflammatory effect of thymol on endometritis, the inflammatory model was established in LPS-induced goat endometrial epithelial cells (gEECs)with thymol treantment. The secretion of NO and cytokines were measured by NO kit and ELISA kits respectively in LPS-induced gEECs. Gene transcription of IL-1β, TLR4, and NF-κB were detected by RT-PCR respectively in LPS-induced gEECs. The results showed that thymol (100, 50, 25 μg·mL^-1) inhibited the secretion of TNF-α, IL-1β, IL-6, NO, and PGE2 to a significant extent in LPS-induced gEECs (P<0.01). In addition, thymol markedly decreased gene transcription of IL-1β, TLR4, and NF-κB in LPS-induced gEECs (P<0.01). These results demonstrated that thymol exerted anti-inflammatory effect through inhibiting the level of inflammatory cytokines, which possibly involved in the inhibition of TLR4/NF-κB signaling pathway.

中图分类号:

S853.74

邵丹, 宋朋杰, 闫宝琪, 武小虎, 王东升, 李亚娟, 张世栋, 严作廷. 百里香酚对脂多糖诱导的子宫内膜上皮细胞炎性反应的抑制作用研究[J]. 畜牧兽医学报, 2019, 50(7): 1493-1499.

SHAO Dan, SONG Pengjie, YAN Baoqi, WU Xiaohu, WANG Dongsheng, LI Yajuan, ZHANG Shidong, YAN Zuoting. Inhibiting Effect of Thymol on Inflammation Induced by LPS in Endometrial Epithelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1493-1499.

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百里香酚对脂多糖诱导的子宫内膜上皮细胞炎性反应的抑制作用研究

Inhibiting Effect of Thymol on Inflammation Induced by LPS in Endometrial Epithelial Cells

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